Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains – PubMed Black Hawk Supplements

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Hericium erinaceus has long been favored for its remarkable nutritional and health-promoting benefits, and erinacine A is the key component responsible for the neuroprotective properties of H. erinaceus. Establishing an efficient method for separating erinacine A from H. erinaceus and screening the erinacine A-enriched strains is crucial to maximizing its benefits. Herein, we first reported that high-speed counter current chromatography (HSCCC) is an effective method for separating high-purity…
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Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains - PubMed

Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains

Mengchen Liu et al. Foods. .

Abstract

Hericium erinaceus has long been favored for its remarkable nutritional and health-promoting benefits, and erinacine A is the key component responsible for the neuroprotective properties of H. erinaceus. Establishing an efficient method for separating erinacine A from H. erinaceus and screening the erinacine A-enriched strains is crucial to maximizing its benefits. Herein, we first reported that high-speed counter current chromatography (HSCCC) is an effective method for separating high-purity erinacine A. Using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (4.5:5:4.5:5, v/v/v/v), erinacine A with a purity of over 95% was separated. Then, we evaluated the content and yield of erinacine A in the liquid-fermented mycelia of Hericium germplasms. Both the content and yield of erinacine A varied greatly among the surveyed strains. The significant effect of the strain on the erinacine A content and yield was revealed by an analysis of variance. The highest erinacine A content and yield were observed in the mycelia of a wild strain HeG, reaching 42.16 mg/g and 358.78 mg/L, which is superior to the current highest outcomes achieved using submerged cultivation. The isolation method established and the strains screened in this study can be beneficial for the scaling up of erinacine A extraction and nutraceutical development to industrial levels.

Keywords: erinacines; high-speed countercurrent chromatography; lion’s mane mushroom; separation and purification; wild germplasm resources.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1

The HSCCC separation chromatogram of extract. The blue area was collected for the HPLC analysis.

Figure 2
Figure 2

The HPLC analysis of extract crude of HeG sample (a), fraction after HSCCC (b) and standard erinacine A (c).

Figure 3
Figure 3

Typical colony morphologies of the investigated Hericium strains.

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Isolation and Evaluation of Erinacine A Contents in Mycelia of Hericium erinaceus Strains – PubMed