Choline Dehydrogenase Contributes to Salt Tolerance in Dunaliella through Betaine Synthesis – PubMed Black Hawk Supplements

BLACK HAWK: Most trusted shilajit supplement for libido

Published article

In Dunaliella tertiolecta, a microalga renowned for its extraordinary tolerance to high salinity levels up to 4.5 M NaCl, the mechanisms underlying its stress response have largely remained a mystery. In a groundbreaking discovery, this study identifies a choline dehydrogenase enzyme, termed DtCHDH, capable of converting choline to betaine aldehyde. Remarkably, this is the first identification of such an enzyme not just in D. tertiolecta but across the entire Chlorophyta. A 3D model of DtCHDH…
Black Hawk Supplements, best supplements in the UK

Choline Dehydrogenase Contributes to Salt Tolerance in Dunaliella through Betaine Synthesis - PubMed

. 2024 Mar-Apr;176(2):e14296.

doi: 10.1111/ppl.14296.

Affiliations

Choline Dehydrogenase Contributes to Salt Tolerance in Dunaliella through Betaine Synthesis

Hao-Hong Chen et al. Physiol Plant. 2024 Mar-Apr.

Abstract

In Dunaliella tertiolecta, a microalga renowned for its extraordinary tolerance to high salinity levels up to 4.5 M NaCl, the mechanisms underlying its stress response have largely remained a mystery. In a groundbreaking discovery, this study identifies a choline dehydrogenase enzyme, termed DtCHDH, capable of converting choline to betaine aldehyde. Remarkably, this is the first identification of such an enzyme not just in D. tertiolecta but across the entire Chlorophyta. A 3D model of DtCHDH was constructed, and molecular docking with choline was performed, revealing a potential binding site for the substrate. The enzyme was heterologously expressed in E. coli Rosetta (DE3) and subsequently purified, achieving enzyme activity of 672.2 U/mg. To elucidate the role of DtCHDH in the salt tolerance of D. tertiolecta, RNAi was employed to knock down DtCHDH gene expression. The results indicated that the Ri-12 strain exhibited compromised growth under both high and low salt conditions, along with consistent levels of DtCHDH gene expression and betaine content. Additionally, fatty acid analysis indicated that DtCHDH might also be a FAPs enzyme, catalyzing reactions with decarboxylase activity. This study not only illuminates the role of choline metabolism in D. tertiolecta’s adaptation to high salinity but also identifies a novel target for enhancing the NaCl tolerance of microalgae in biotechnological applications.

PubMed Disclaimer

Similar articles

References

REFERENCES

    1. Aleksenko V. A., Anand D., Remeeva A., Nazarenko V. V., Gordeliy V., Jaeger K.‐E., Krauss U., Gushchin I. (2020) Phylogeny and Structure of Fatty Acid Photodecarboxylases and Glucose‐Methanol‐Choline Oxidoreductases. Catalysts 10(9)
    1. Apse M. P., Blumwald E. (2002) Engineering salt tolerance in plants. Current Opinion in Biotechnology 13(2), 146–150
    1. Ben‐Amotz A., Avron M. (1992) Dunaliella: physiology, biochemistry, and biotechnology. CRC press
    1. Breisch J., Bendel M., Averhoff B. (2022) The choline dehydrogenase BetA of Acinetobacter baumannii: a flavoprotein responsible for osmotic stress protection. Environmental Microbiology 24(3), 1052–1061
    1. Changjie J., Zhengwei L., Xianzhi X. (2023) Priming for Saline‐Alkaline Tolerance in Rice: Current Knowledge and Future Challenges. Rice Science 30(5), 417–425

MeSH terms

Substances

Grants and funding

LinkOut – more resources

BLACK HAWK: Best shilajit supplement for women

Read the original publication:

Choline Dehydrogenase Contributes to Salt Tolerance in Dunaliella through Betaine Synthesis – PubMed