Histopathological and immunohistochemical investigation of the effect of Shilajit in rats with experimental spinal cord injury – PubMed Black Hawk Supplements

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CONCLUSION: It is thought that the use of Shilajit in SCI will reduce the effects of secondary damage in SCI and that its administra-tion to such patients will have positive effects on the results.
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Histopathological and immunohistochemical investigation of the effect of Shilajit in rats with experimental spinal cord injury - PubMed

Histopathological and immunohistochemical investigation of the effect of Shilajit in rats with experimental spinal cord injury

Eyüp Çetin et al. Ulus Travma Acil Cerrahi Derg. 2023 Dec.

Abstract

Background: This experimental study was designed to investigate the histopathological and immunohistochemical effects of Shilajit in rats with experimentally induced spinal cord injury (SCI).

Methods: The rats were divided into three groups: Control group: The group in which spinal cord damage was created but no drug was administered. Low-dose group: This is the group in which intraperitoneal Shilajit is given at a dose of 150 mg/kg at the 1st h, 1st day, 2nd day, and 3rd day after spinal cord damage was induced. High-dose group: This is the group in which intraperitoneal Shilajit is given at a dose of 250 mg/kg at the 1st h, 1st day, 2nd day, and 3rd day after spinal cord damage was induced. Thin sections taken from the spinal cord after euthanasia were sent for histopathological and immunohistochemical examination.

Results: Histopathological examination of the high-dose group showed lower amounts of morphological findings compared to the low-dose group and control group. While a significant CD68 immune reaction was observed in the control group of rats with spinal injury, the positive immune reaction was found to be significantly decreased in the Shilajit-applied groups.

Conclusion: It is thought that the use of Shilajit in SCI will reduce the effects of secondary damage in SCI and that its administra-tion to such patients will have positive effects on the results.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1

(a,b,c): Showing experimentally induced-spinal injury in rats.

Figure 2
Figure 2

Cross-section of the medulla spinalis stained with hematoxylin and eosin (H&E): (a,b) Group I (Control; Spinal Injury): In the area of spinal injury, a marked inflammatory reaction consisting of vascular proliferation, macrophages and neutrophil leukocytes is observed. (c,d) Group II (Spinal Injury + 150 mg/kg Shilajit): Mild leukocyte infiltration and degeneration are observed in the spinal cord. (e,f) Group III (Spinal Injury + 250 mg/kg Shilajit): Almost similar findings observed in group II are also observed in this group.

Figure 3
Figure 3

CD68 immunolocalization in cross-sections of the rat medulla spinalis. (a) Group I (Spinal Injury) showing strong nuclear expression of CD68. (b) Group II (Spinal Injury + 150 mg/kg Shilajit) showing weak nuclear CD68 immunoreactivity. (c) Group III (Spinal Injury + 250 mg/kg Shilajit) showing less CD68 immunoreactivity compared to the other two groups.

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References

    1. Warner FM, Cragg JJ, Jutzeler CR, Finnerup NB, Werhagen L, Weidner N, et al. Progression of neuropathic pain after acute spinal cord injury:A meta-analysis and framework for clinical trials. J Neurotrauma. 2018;36:1461–8. – PubMed
    1. Hamid R, Averbeck MA, Chiang H, Garcia A, Al Mousa RT, Oh SJ, et al. Epidemiology and pathophysiology of neurogenic bladder after spinal cord injury. World J Urol. 2018;36:1517–27. – PubMed
    1. Tsintou M, Dalamagkas K, Seifalian AM. Advances in regenerative therapies for spinal cord injury:A biomaterials approach. Neural Regen Res. 2015;10:726–42. – PMC – PubMed
    1. McDaid D, La Park A, Gall A, Purcell M, Bacon M. Understanding and modelling the economic impact of spinal cord injuries in the United Kingdom. Spinal Cord. 2019;57:778–88. – PMC – PubMed
    1. Zheng B, Tuszynski MH. Regulation of axonal regeneration after mammalian spinal cord injury. Nat Rev Mol Cell Biol. 2023;24:396–413. – PubMed

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Histopathological and immunohistochemical investigation of the effect of Shilajit in rats with experimental spinal cord injury – PubMed