Nutritional vitamin B12 regulates RAS/MAPK-mediated cell fate decisions through one-carbon metabolism – PubMed Black Hawk Supplements

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Vitamin B12 is an essential nutritional co-factor for the folate and methionine cycles, which together constitute one-carbon metabolism. Here, we show that dietary uptake of vitamin B12 modulates cell fate decisions controlled by the conserved RAS/MAPK signaling pathway in C. elegans. A bacterial diet rich in vitamin B12 increases vulval induction, germ cell apoptosis and oocyte differentiation. These effects are mediated by different one-carbon metabolites in a tissue-specific manner. Vitamin…
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Nutritional vitamin B12 regulates RAS/MAPK-mediated cell fate decisions through one-carbon metabolism - PubMed

Nutritional vitamin B12 regulates RAS/MAPK-mediated cell fate decisions through one-carbon metabolism

Ana Cristina Laranjeira et al. Nat Commun. .

Abstract

Vitamin B12 is an essential nutritional co-factor for the folate and methionine cycles, which together constitute one-carbon metabolism. Here, we show that dietary uptake of vitamin B12 modulates cell fate decisions controlled by the conserved RAS/MAPK signaling pathway in C. elegans. A bacterial diet rich in vitamin B12 increases vulval induction, germ cell apoptosis and oocyte differentiation. These effects are mediated by different one-carbon metabolites in a tissue-specific manner. Vitamin B12 enhances via the choline/phosphatidylcholine metabolism vulval induction by down-regulating fat biosynthesis genes and increasing H3K4 tri-methylation, which results in increased expression of RAS/MAPK target genes. Furthermore, the nucleoside metabolism and H3K4 tri-methylation positively regulate germ cell apoptosis and oocyte production. Using mammalian cells carrying different activated KRAS and BRAF alleles, we show that the effects of methionine on RAS/MAPK-regulated phenotype are conserved in mammals. Our findings suggest that the vitamin B12-dependent one-carbon metabolism is a limiting factor for diverse RAS/MAPK-induced cellular responses.

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References

    1. Bose, S., Allen, A. E. & Locasale, J. W. The molecular link from diet to cancer cell metabolism. Mol. Cell 78, 1034–1044 (2020). – PubMed – PMC – DOI
    1. Green, R. et al. Vitamin B12 deficiency. Nat. Rev. Dis. Primers. 3, 17041 (2017).
    1. Watson, E. et al. Interspecies systems biology uncovers metabolites affecting C. elegans gene expression and life history traits. CELL 156, 759–770 (2014). – PubMed – PMC – DOI
    1. Watson, E. et al. Metabolic network rewiring of propionate flux compensates vitamin B12 deficiency in C. elegans. eLife 5, 1–21 (2016). – DOI
    1. Ducker, G. S. & Rabinowitz, J. D. One-carbon metabolism in health and disease. Cell Metab. 25, 27–42 (2017). – PubMed – DOI

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Nutritional vitamin B12 regulates RAS/MAPK-mediated cell fate decisions through one-carbon metabolism – PubMed