Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods – PubMed Black Hawk Supplements

BLACK HAWK: High quality lions mane supplement for stress

Published article

Determining the content of the nutrient choline in foods and obtaining the required amount from the diet are crucial. One way to measure choline in foods is by converting choline esters to free choline via acid hydrolysis, followed by quantifying the total choline, as adopted by the AOAC method (AOAC-Choline); however, certain choline esters are difficult to hydrolyse. Here, we investigated various acid hydrolysis conditions to establish a reliable method for determining the total choline in…
Black Hawk Supplements, best supplements in the UK

Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods - PubMed

Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods

Yoshinari Hirakawa et al. Sci Rep. .

Abstract

Determining the content of the nutrient choline in foods and obtaining the required amount from the diet are crucial. One way to measure choline in foods is by converting choline esters to free choline via acid hydrolysis, followed by quantifying the total choline, as adopted by the AOAC method (AOAC-Choline); however, certain choline esters are difficult to hydrolyse. Here, we investigated various acid hydrolysis conditions to establish a reliable method for determining the total choline in foods by detecting free choline using highly sensitive and selective mass spectrometry. Hydrolysis in 0.055 mol/L HCl for 8 h in an autoclave (121 °C) was found to be optimal for the hydrolysis of choline esters in various foods. Twenty-four foods, including grains, seed, vegetables, fruits, mushroom, algae, fish, meats, beverage, processed foods, and egg, were measured. The trends in the total choline content were consistent with previous reports; however, the choline content was 10-20% higher than that measured using AOAC-Choline. Therefore, re-evaluation of the total choline content in foods using our constructed method is recommended. This reassessment will allow for a more reliable determination of choline intake for maintaining health.

Keywords: Acid hydrolysis; Choline; Choline esters; Liquid chromatograph-tandem mass spectrometry; Total choline; Various foods.

PubMed Disclaimer

References

    1. Zeisel, S. H. & Blusztajn, J. K. Choline and human nutrition. Annu. Rev. Nutr. 14, 269–296 (1994). – DOI – PubMed
    1. Zeisel, S. H. & da Costa, K. A. Choline: An essential nutrient for public health. Nutr. Rev. 67(11), 615–623 (2009). – DOI – PubMed
    1. Blusztajn, J. K. Choline, a vital amine. Science. 281(5378), 794–795 (1998). – DOI – PubMed
    1. Institute of Medicine. Dietary reference intakes for thiamine, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline. http://www.ncbi.nlm.nih.gov/books/NBK114310/ (1998).
    1. Koc, H., Mar, M. H., Ranasinghe, A., Swenberg, J. A. & Zeisel, S. H. Quantitation of choline and its metabolites in tissues and foods by liquid chromatography/electrospray ionization-isotope dilution mass spectrometry. Anal. Chem. 74(18), 4734–4740 (2002). – DOI – PubMed

MeSH terms

Substances

BLACK HAWK: Best shilajit supplement for teens

Read the original publication:

Optimisation of acid hydrolysis conditions of choline esters and mass spectrometric determination of total choline in various foods – PubMed