Targeting Neuroinflammation and Apoptosis: Cardamonin's Cognitive Benefits in Alzheimer's 5XFAD Mice – PubMed Black Hawk Supplements

BLACK HAWK: High quality shilajit supplement for adrenal fatigue

Published article

This study aimed to evaluate the cognitive-enhancing and neuroprotective effects of cardamonin in the 5XFAD transgenic mouse model of Alzheimer’s disease (AD). We treated six-month-old female 5XFAD mice with cardamonin at 5 mg/kg, 10 mg/kg, and 20 mg/kg. Cognitive function was assessed using the Morris Water Maze (MWM) and Novel Object Recognition (NOR) tests. ELISA, western blot, and PCR analyses evaluated amyloid-beta (Aβ) levels, neuroinflammation markers, and apoptosis-related factor…
Black Hawk Supplements, best supplements in the UK

Targeting Neuroinflammation and Apoptosis: Cardamonin's Cognitive Benefits in Alzheimer's 5XFAD Mice - PubMed

Targeting Neuroinflammation and Apoptosis: Cardamonin’s Cognitive Benefits in Alzheimer’s 5XFAD Mice

Shukur Wasman Smail. Neurochem Res. .

Abstract

This study aimed to evaluate the cognitive-enhancing and neuroprotective effects of cardamonin in the 5XFAD transgenic mouse model of Alzheimer’s disease (AD). We treated six-month-old female 5XFAD mice with cardamonin at 5 mg/kg, 10 mg/kg, and 20 mg/kg. Cognitive function was assessed using the Morris Water Maze (MWM) and Novel Object Recognition (NOR) tests. ELISA, western blot, and PCR analyses evaluated amyloid-beta (Aβ) levels, neuroinflammation markers, and apoptosis-related factor expression. All animals survived without toxicity. Cardamonin treatment significantly improved spatial learning and memory retention in MWM and NOR tests, with the 20 mg/kg dose showing the most pronounced effects. Additionally, cardamonin reduced soluble and insoluble Aβ levels in the frontal cortex and hippocampus. The treatment also significantly decreased neuroinflammatory markers, with IL-1β, IL-6, and TNF-α levels dropping substantially at higher doses. Cardamom treatment also normalizes cleaved caspase 3, GFAP, Iba-1, PSD-95, and synaptophysin, which aids in restoring synaptic integrity. Furthermore, cardamonin led to a marked reduction in apoptosis-related gene expression, indicating its potential to mitigate neurodegeneration. Cardamonin demonstrates significant cognitive-enhancing and neuroprotective properties in the 5XFAD mouse model, suggesting its potential as a therapeutic agent for AD. These findings support further investigation into cardamonin’s mechanisms and applicability in treating neurodegenerative disorders.

Keywords: 5XFAD mice; Alzheimer’s disease; Aβ plaques; Cardamonin; Memory; Neuroinflammation.

PubMed Disclaimer

Conflict of interest statement

Declarations. Competing Interests: The authors declare no competing interests.

References

    1. Saleem F, Naz F, Khan KM (2023) Patent analysis of novel therapeutic approaches for treating Alzheimer’s disease 2018–2023. In. Taylor & Francis, pp 91–94
    1. Shoghi-Jadid K, Small GW, Agdeppa ED, Kepe V, Ercoli LM, Siddarth P, Read S, Satyamurthy N, Petric A, Huang S-C (2002) Localization of neurofibrillary tangles and beta-amyloid plaques in the brains of living patients with Alzheimer disease. Am J Geriatric Psychiatry 10:24–35 – DOI
    1. Armstrong RA (2009) The molecular biology of senile plaques and neurofibrillary tangles in Alzheimer’s disease. Folia Neuropathol 47:289–299 – PubMed
    1. Yin X, Qiu Y, Zhao C, Zhou Z, Bao J, Qian W (2021) The role of amyloid-beta and tau in the early pathogenesis of Alzheimer’s Disease. Med Sci Monitor: Int Med J Experimental Clin Res 27:e933084–e933081 – DOI
    1. Luan K, Rosales JL, Lee K-Y (2013) Crosstalks between neurofibrillary tangles and amyloid plaque formation. Ageing Res Rev 12:174–181 – DOI – PubMed

MeSH terms

Substances

BLACK HAWK: Best shilajit supplement for mums

Read the original publication:

Targeting Neuroinflammation and Apoptosis: Cardamonin's Cognitive Benefits in Alzheimer's 5XFAD Mice – PubMed