Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF) – PubMed Black Hawk Supplements

BLACK HAWK: High quality ashwagandha supplement for athletic performance

Published article

Pathogenesis-related proteins (PR), including osmotins, play a vital role in plant defense, being activated in response to diverse biotic and abiotic stresses. Despite their significance, the mechanistic insights into the role of osmotins in plant defense have not been extensively explored. The present study explores the cloning and characterization of the osmotin gene (WsOsm) from Withania somnifera, aiming to illuminate its role in plant defense mechanisms. Quantitative real-time PCR analysis…
Black Hawk Supplements, best supplements in the UK

Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF) - PubMed

. 2024 Sep-Oct;176(5):e14513.

doi: 10.1111/ppl.14513.

Affiliations

Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF)

Varinder Singh et al. Physiol Plant. 2024 Sep-Oct.

Abstract

Pathogenesis-related proteins (PR), including osmotins, play a vital role in plant defense, being activated in response to diverse biotic and abiotic stresses. Despite their significance, the mechanistic insights into the role of osmotins in plant defense have not been extensively explored. The present study explores the cloning and characterization of the osmotin gene (WsOsm) from Withania somnifera, aiming to illuminate its role in plant defense mechanisms. Quantitative real-time PCR analysis revealed significant induction of WsOsm in response to various phytohormones e.g. abscisic acid, salicylic acid, methyl jasmonate, brassinosteroids, and ethrel, as well as biotic and abiotic stresses like heat, cold, salt, and drought. To further elucidate WsOsm’s functional role, we overexpressed the gene in Nicotiana tabacum, resulting in heightened resistance against the Alternaria solani pathogen. Additionally, we observed enhancements in shoot length, root length, and root biomass in the transgenic tobacco plants compared to wild plants. Notably, the WsOsm- overexpressing seedlings demonstrated improved salt and drought stress tolerance, particularly at the seedling stage. Confocal histological analysis of H2O2 and biochemical studies of antioxidant enzyme activities revealed higher levels in the WsOsm overexpressing lines, indicating enhanced antioxidant defense. Furthermore, a pull-down assay and mass spectrometry analysis revealed a potential interaction between WsOsm and defensin, a known antifungal PR protein (WsDF). This suggests a novel role of WsOsm in mediating plant defense responses by interacting with other PR proteins. Overall, these findings pave the way for potential future applications of WsOsm in developing stress-tolerant crops and improving plant defense strategies against pathogens.

Keywords: Gene Expression; Osmotin; Pathogenesis‐related proteins; Plant defense; Reactive oxygen species.

PubMed Disclaimer

References

REFERENCES

    1. Allen, G. C., Flores‐Vergara, M. A., Krasynanski, S., Kumar, S. & Thompson, W. F. (2006). A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nature Protocols, 1(5), 2320–2325. https://doi.org/10.1038/nprot.2006.384.
    1. Anzlovar, S. & Dermastia, M. (2003). The comparative analysis of osmotins and osmotin‐like PR‐5 proteins. Plant Biology, 5(02), 116–124. https://doi.org/10.1055/s-2003-40723.
    1. Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiology, 24(1), 1–15. https://doi.org/10.1104/pp.24.1.1.
    1. Bashir, M. A., Silvestri, C., Ahmad, T., Hafiz, I. A., Abbasi, N. A., Manzoor, A., Cristofori, V. & Rugini, E. (2020). Osmotin: A cationic protein leads to improve biotic and abiotic stress tolerance in plants. Plants, 9(8), 992. https://doi.org/10.3390/plants9080992
    1. Choudhury, F. K., Rivero, R. M., Blumwald, E. & Mittler, R. (2017). Reactive oxygen species, abiotic stress and stress combination. The Plant Journal, 90(5), 856–867. https://doi.org/10.1111/tpj.13299.

MeSH terms

Substances

Grants and funding

BLACK HAWK: Best ashwagandha supplement for dads

Read the original publication:

Withania somnifera osmotin (WsOsm) confers stress tolerance in tobacco and establishes novel interactions with the defensin protein (WsDF) – PubMed